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chameleon duo pre stained protein ladder (LI-COR)

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LI-COR chameleon duo pre stained protein ladder
Chameleon Duo Pre Stained Protein Ladder, supplied by LI-COR, used in various techniques. Bioz Stars score: 96/100, based on 421 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+ladder/CHAMELEON+DUO+PRE-STAINED+PROTEIN+LADDER/pmc13178796-88-4-9
Average 96 stars, based on 421 article reviews

chameleon duo pre stained protein ladder - by Bioz Stars, 2026-09

96/100 stars

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Staining:

Article Title: Acute Intermittent Hypoxia Exerts Beneficial Effects and Promotes Repair in Male Mice in the Experimental Autoimmune Encephalomyelitis Model of Multiple Sclerosis.
Article Snippet: Extracted proteins from samples were separated by SDS- PAGE (Bio- Rad 10- well, 50 μL, 10% Mini- PROTEAN TGX Stain- Free Precast Gels #4568034). .. Protein ladder (4 μL; Li- Cor Chameleon Duo Pre- Stained Ladder #928- 60000) and 20 μL of each sample were then loaded into respective wells and the gels were run at 125 V for 1 h and 15 min in Tris- glycine running buffer. .. Gels were viewed under a BioRad ChemiDoc imaging system for UV activation to image total protein prior to semi- dry transfer (Bio- Rad Trans- Blot SD SemiDry Transfer Cell) to PVDF membrane (Bio- Rad Immun- Blot PVDF Membrane #1620177).

Nucleic Acid Electrophoresis:

Article Title: FSP1 and histone deacetylases suppress cancer persister cell ferroptosis
Article Snippet: Lysates were combined with sample buffer (Thermo Fisher Scientific, NP0007) and incubated at 70°C for 10 min. .. Samples were run on SDS–polyacrylamide gel electrophoresis gels (Bolt 4 to 12% Bis-Tris Gel, Life Technologies, NW04120BOX), with the Chameleon Duo Pre-stained Protein Ladder (LI-COR, #928-60000), and transferred to a nitrocellulose membrane using the iBLOT 2 Dry Blotting System (Life Technologies, IB21001). .. Membranes were blocked with 5% bovine serum albumin (GeminiBio, 700-100P-1KG) for 1 hour at room temperature before overnight incubation at 4°C.

Membrane:

Incubation:

Article Title: Electrostatic control of chromatin compaction safeguards against apoptotic DNA release
Article Snippet: The pellet was resuspended in 100 μL 6x Laemmli buffer (375 mM Tris pH 6.8 (Sigma); 12% SDS w/w (Serva); 60% Glycerol w/w (Sigma); 0.06% Bromophenol blue (Merck)), supplemented with 60 mM DTT (Sigma) and 1 U Benzonase (Merck). .. Samples were incubated at room temperature for 5 min, then boiled at 95°C for 10 min. 3.5 μL of each sample was loaded in 10-well 1.0 mm 4-12% NuPage Bis-TRIS gels (Thermo), alongside 3.5 μL ChameleonTM Duo Pre-stained Protein Ladder (Li-COR, 928-60000). .. Gels were run in MES SDS Running Buffer (Thermo) at 120 V for 10 min, then at 200 V for 20 min. Gels were removed and equilibrated in Transfer Buffer (20% ethanol w/w (Sigma); 10% 10X Transfer Buffer (Thermo)) for 10 min. Proteins were transferred onto 0.2 μm AmershamTM Protran nitrocellulose membranes (Cytiva, 10600001) using the Mini Trans-Blot® Cell system (BioRad).